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KMID : 0371619980140010037
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Journal of Wonkwang Medical Science
1998 Volume.14 No. 1 p.37 ~ p.44
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Security test for fluorescein staining in fluorescein leukocyte angiography and separation of fluorescein lymphocyte
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Kim Yun-Hee
Yang Yun-Sik
Kim Jae-Duck
Kang Pil-Sung
Kim Kang-Ju
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Abstract
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Recently, retinal blood flow has been studied with several methods, targeted dye delivery method, fluorescent vesicle system, laser Doppler velocimetry, acridine orange staining method. Authors presented a new method, fluorescein leukocyte angiography using a scanning laser ophthalmoscope which include external staining of leukocyte with fluorescein dye and reintroduce the fluorescent leukocyte into the blood flow.
Authors tried to check security of external fluorescein staining of leukocytes and separation of fluorescent lymphocytes from whole blood and supernatant fluid using Ficoll-Paque, with cell viability, leukocyte recovery rate and erythrocyte contamination rate.
There was no significant difference in cell viability, leukocyte recovery rate and erythrocyte contamination rate between fluorescein staining group and non-staining group. Fluorescent lymphocytes could be separated from whole blood and supernatant solution after gravity sedimentation with 6% hetastarch and the gravity sedimented group had low erythrocyte contamination rate than whole blood group(p≤0.05).
In conclusion, external fluorescein staining procedure of leukocyte during fluorescein leukocyte angiography could be performed without cell injury compared to non-fluorescein staining group and fluorescent lymphocyte could be separated from whole blood and supernatant fluid after gravity sedimentation.
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KEYWORD
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fluorescein leukocyte angiography, cell viability test, fluorescein lymphocyte angiography, scanning laser ophthalmoscope
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